Animal Health Laboratory

Animal Health Division Supplies//Testing Services and Fees//Submissions/Submission Protocol


Please note: For the remainder of 2020, We are closed September 7th, October 5th, November 11th, November 26th, November 27th and December 25th. If unsure of closures, before sending samples, please call the lab to verify any other closures. Thank you.

We are open Monday through Friday 8am to 5pm

The Animal Health Lab is an ISO 17025 accredited laboratory that provides fast and accurate lab results. We offer testing services for regulatory diseases including Brucellosis, Johne's, Trichomoniasis, BVD, Equine Infectious Anemia (EIA), and Scrapie Genetic Susceptibility.

Happening Now

EIA Mobile App is now available! 

  • Go to your App Store or Play Store and search for "USALIMS".  Once downloaded call the lab to request a username and password.  Please note:  This app is currently only for submitting equine serum samples to AHL for EIA testing.  If using another laboratory for your EIA testing needs call that laboratory to inquire about obtaining a username and password from them directly for the EIA mobile app.

2020 - 2021 Trich Tags

  • Trich tag color for October 1, 2020 to September 30, 2021 is GREEN.  Any green tags you have purchased in previous years can be used now.  Call (303) 869-9230 now to get your trich tags and tubes ($7.50/tube) ordered.

News

The lab moved April 2019 to Broomfield!

AHL Lab

Important notices about Trich testing
  • AHL offers Pooled Trich Testing.  The cost is $35.00 per pool of 5 samples.  You will still need to collect the individual samples in InPouches or Trich Transit Tubes as pooling is conducted only in the laboratory.  Please note, if any of the pooled samples are found to be positive, PCR will be done on each sample in the pool at a cost of $25.00 per sample.
  • If using Pooled PCR and traveling out of state, please make sure the state to which the bulls are traveling to accepts Pooled Trich PCR sample results.
  • Any Trich InPouch or Trich Transit Tube samples requesting PCR or Pooled PCR testing must be received within 72 hours of collection.
  • Although AHL advises against using Lactated Ringers (LRS), if used, the sample must be received within 24 hours of collection and will be transferred to either an InPouch or Transit Tube (depending on requested test) upon arrival, and the submitting veterinarian will be charged the cost of the media as well as a transfer fee.  EXPIRED POUCHES AND TUBES WILL NOT BE ACCEPTED.
  • Once samples are received in the laboratory, they become the property of the laboratory and will not be returned to the client for any reason.
Disease Information and Brochures

 

Below are some resource materials for just a few of the diseases tested at the Rocky Mountain Regional Animal Health Laboratory. Each of the brochures are available for download in a PDF format.

Bovine Leukosis Virus poster

Bovine Leukosis Virus (BLV)

Bovine leukosis virus is a retrovirus infection of dairy and beef cattle. The virus survives in white blood cells, called lymphocytes. Only about 5% of the cows infected with this blood-borne disease ever develop a clinical disease. If clinical disease is not present, BLV infection may be diagnosed by blood tests (AGID and ELISA) that detect the presence of BLV antibodies. BLV infection does not occur naturally in other species. BLV infection is for life; once an animal is infected, it is virus positive for life.

Bovine Leukosis Virus


CAE

Caprine Arthritis Encephalitis Virus (CAEV)

Caprine Arthritis Encephalitis Virus is a disease of goats. Those infected with CAEV may develop arthritis, mastitis, encephalitis, and/or pneumonia. The primary route of transmission is through ingestion of virus-infected colostrum or milk by kids. Contact with the blood of an infected animal can also transmit the disease. Currently there is no vaccine available for CAEV.

Caprine Arthritis Encephalitis Virus


Johne's

Johne's (Mycobacterium avium subsp. paratuberculosis) Disease

Johne's Disease is a chronic intestinal disorder primarily of ruminants. Symptoms include decreased milk productivity, diarrhea and weight loss despite a good appetite. A recent survey of Colorado dairy cattle found a 4% seroprevalance of Johne's Disease among cattle at least 2 years of age (JAVMA 2004 Jul 1, 225(1): 97-101).

Johne's Disease


Scrapie Testing

Scrapie

Scrapie is a fatal nervous system disease of sheep and goats and is caused by the same family of infectious agents as Mad Cow Disease. Scrapie is a major concern to the sheep and goat industry, causing significant production losses. Moreover, the presence of scrapie in the United States also limits the export of breeding stock, semen, and embryos.

Scrapie Susceptibility Testing

 

Trich Brochure

Trichomoniasis

Trichomoniasis is a venereal disease of cattle caused by the protozoan parasite Trichomonas foetus. Bovine trichomoniasis is spread by breeding and causes infertility, abortions, and occasionally chronic uterine infection in dairy and beef cattle. Losses include reduced milk production, reduced calf crop, and delayed breeding. Diagnosis of the disease requires demonstration of the organism in the tissue or most frequently in culture. There is not a treatment for Bovine trichomoniasis.

Bovine Trichomoniasis

Supplies

Supplies are available from the Colorado Department of Agriculture  Animal Health Laboratory to accredited veterinarians. Some quantities may be limited and all prices are subject to change. Please let us know in advance if you have a large order to place so that we may have the full quantity of the item you need.

Orders need to be placed by 1:00 pm to be sent out same day.

AHL Supplies

 

Supplies can be ordered by phone, fax, or email
Phone: (303) 869-9230                         
Fax: (303) 869-9232                          

AHL also supplies Tuberculin, EIA Test Records, Brucellosis Test Records and more!  Call us to find out more information.

Testing Services and Fees

There is a 24/7 sample drop box located at the northeast corner of the building.  Please place samples in a cardboard box before placing in the drop box.  If left in a bag samples may break.

When sending samples via FedEx, UPS or USPS please package samples carefully so they are not damaged in transit. Submissions to the laboratory should comply with applicable biosafety regulations. Submissions to the laboratory must be accompanied by a proper submission form for all samples except EIA mobile submissions.

Testing charges will be billed to the veterinarian submitting the samples. There is no additional charge for samples originating from out-of-state.

Testing Services and Fees

Submissions

 A sample submission form, VS 10-11 or VS 4-33 must accompany all samples tested at the Animal Health Laboratory (unless using the LIMS portal or EIA Mobile App). The chart must contain the following information.

  • Veterinarian's Name
  • Date sample was taken
  • Owners Name
  • Physical Address of Animals
  • Type of test to be run
  • Recorded tube numbers of the animals being tested that has been matched up to the sample taken

Any information missing can lead to a delay in your test results being finished. Please take the time to check all required information is on the submission form before mailing samples in.

Picture of Sample Submission Form

The Animal Health Lab offers a general sample submission form that is to be used for general serology for Anaplasmosis, Bluetongue, Bovine Leukosis, Brucella canis, Brucella ovis, Caprine Arthritis Encephalitis, Johne's Disease, Pseudorabies, Scrapie Genotyping (unofficial test only), and Tritrichomonas foetus.  A VS 10-11 form (unless using the EIA Mobile App) must accompany EIA samples and a VS 4-33 must accompany all official Brucella abortus, Brucella suis and Brucella melitensis testing.

Sample Submission Form

USDA Forms are now available at AHL, give us a call at 303-869-9230

    Submission Protocol

    The following submission protocols are provided so that the highest quality sample can be obtained. Collecting the sample the right way the first time can save a lot of time and hassle. Following the protocols will help to ensure that samples are of sufficient volume and quality for testing.

    B. ovis Semen PCR Submission Protocol

    Johne's Fecal PCR Submission Protocol

    Sample Submission Protocol

    • Use this protocol when submitting samples for serological testing such as Anaplasmosis, Brucella abortus, B. canis, B. melitensis, B. ovis, Bluetongue, Bovine Leukosis, Caprine Arthritis Encephalitis, Equine Infectious Anemia, and Pseudorabies.
    • General Serology Submission Protocol

    Scrapie Genotyping EDTA Blood Collection Protocol

    Scrapie Genotyping FTA Collection Protocol

    Trichomonas Foetus Sample Collection Protocol

    FAQs

    Questions Regarding Supplies

    Q:   Where do I get Tuberculin?

    A:   Right here at the AHL, just give us a call at 303-869-9230.

    Q:   Where do I get Equine Infectious Anemia (EIA) submissions forms?

    A:   Right here at the AHL, just give us a call at 303-869-9230.

    Q:   Where do I get OCV (orange) and Bright (silver) tags?

    A:   The Animal Health Laboratory supplies both of these tags. Please call 303-869-9230 to order these items.

    Q:   Where do I get books of Interstate Certificates of Veterinary Inspection?

    A:   The Animal Health Laboratory still supplies books of Interstate Certificates of Veterinary Inspection for $75/book of 25 certificates. Please call 303-869-9230 to order.  If you are interested in using electronic certificates of veterinary inspection call the Colorado State Vet's Office at 303-869-9130.

    Questions Regarding Sample Submission

    Q:   How much blood do I need for testing?

    A:   Please provide at least 2 ml of serum for serological tests. If you are requesting multiple tests only one tube with 2 ml of serum is generally necessary to handle all of the tests requested.

    Q:   What kind of tube can I use?

    A:   For most of the serological testing done here, any red top or serum separator tube, 10 cc or less is acceptable.

    If you are submitting for Scrapie Genetic Susceptibility testing only whole blood purple top tubes with EDTA are acceptable.

    Q:   Where do I ship my EVA samples?

    A:   Please send your samples to the Colorado State University Veterinary Diagnostic Laboratory in Fort Collins. If you have any questions regarding EVA testing please contact CSU directly at (970) 297-1281

    Q:   How long can I expect to wait for results?

    A:   Many of the tests the Animal Health Laboratory performs can be completed the same day provided samples are to the laboratory by noon. Same day results mean they will be finished and reported out by 4:30 pm. Some notable exceptions to this are any AGID, Scrapie Susceptibility, and Trichomonas testing. If you need results immediately, please contact the AHL at 303-869-9230 to discuss your options. For a complete schedule of our tests please visit our Testing Services and Fees page.

    Questions Regarding Public Health Issues

    Q:   What do I do if I've found a dead squirrel or bird?

    A:   The Animal Health Laboratory does not handle any type of animals directly. Please contact the Colorado Department of Public Health regarding any dead animal you suspect of having died of suspicious causes. Their animal helpline is (877) 462-2911.

    Contact Us

    Tiffany Brigner
    Laboratory Manager
    AHL
    300 S. Technology Ct. Broomfield CO 80021
    Phone number: (303) 869-9230
    Fax number: (303) 869-9232


    Additional Resources 

    CSU logo


    CSU Diagnostic Laboratory

    A2LA Accreditation

    Scope Of Accreditation To ISO/IEC 17025:2005

    A2LA Accreditation

    The Colorado Department of Agriculture's Animal Health Laboratory (CDA-AHL) is accredited to ISO/IEC 17025:2005 and the OIE Quality Standard and Guidelines for Veterinary Laboratories: Infectious Disease, 2008 through the American Association for Laboratory Accreditation (A2LA). These internationally recognized standards set stringent requirements for operating quality systems in testing laboratories.

    If you would like to you can view the AHL's Certificate of Accreditation.

    If you would like to find out additional information on A2LA's accreditation program please visit A2LA.

     

    A2LA accredited symbol

    In January 2006, the Colorado Department of Agriculture's Animal Health Laboratory (CDA-AHL) gained an elite accreditation from the American Association for Laboratory Accreditation (A2LA). The Animal Health Lab attained ISO/IEC 17025-2005 accreditation which is an internationally recognized standard that sets stringent requirements for operating quality systems in testing laboratories.  Every other year the lab participates in an on-site assessment to maintain this accreditation.