Animal Health Laboratory

Animal Health Division

Supplies//Testing Services and Fees//Submissions/Submission Protocol

The Animal Health Lab is an ISO 17025 accredited laboratory that provides fast and accurate lab results. We offer testing services for regulatory diseases including Brucellosis, Johne's, Trichomoniasis, BVD, and Equine Infectious Anemia (EIA).

We are open Monday through Friday 8 am to 5 pm.

In 2023, we will be closed on:

  • Jan 2 - New Year's Day
  • Jan 16 - MLK Jr Day
  • Feb 20 - Presidents' Day
  • May 29 - Memorial Day
  • June 19 - Juneteenth
  • Jul 3 - Governor's Holiday
  • Jul 4 - Independence Day
  • Sep 4 - Labor Day
  • Oct 2 - Francis Cabrini Day
  • Nov 10 - Veterans Day
  • Nov 23 - Thanksgiving
  • Dec 25 - Christmas Day

If unsure of laboratory closures, before sending samples please call the lab at 303-869-9230, or email us at cda_ahlab@state.co.us to verify any other closures. Thank you.

Happening Now

  • NEW! We've created an easy to use electronic spreadsheet to use for submission information. See the Submissions section below.
  • NEW! General AHL email: cda_ahlab@state.co.us
  • Call or email the lab to get set up on the EIA Mobile App.
  • 2022 - 2023 Trich Tags
    • Trich tag color for October 1, 2022 to September 30, 2023 is ORANGE. Any orange tags you have purchased in previous years can be used now.

Trich Testing Information

  • Individual and Pooled RT-PCR testing available. If testing bulls to move out of state, please make sure the destination state accepts RT-PCR (individual and/or pooled testing) results.
  • Cost:
    • Pooled testing: $8 per sample (2-5 samples per pool) with a minimum charge of $24 per pool. Collect the individual samples in acceptable media and the lab will pool.
    • Individual testing: $30 per sample
    • If any of the pooled samples are found to be positive, individual RT-PCR will be done on each sample in the pool and you will be charged for both pooled and individual testing on the affected pool.
  • Accepted media types:  phosphate-buffered saline (PBS), sterile saline, LRS, BioMed InPouch and TF Transit Tubes, and Diamond's media
  • Samples can be received up to 144 hours (6 days) after collection. Please continue to ship the samples to the lab as soon as possible after collection to allow for shipping issues and delays. If necessary, refrigerate samples until shipment.
  • Please legibly label tubes with veterinarian and owner name (initials are fine), and bull identification numbers
  • Complete a submission document, see Submissions (<---link to Submissions section) 
    • All animals must have official ID (i.e. 840 or 84 tag)
    • CO Trich tag IDs are required only if bulls are staying in CO
  • Media:
    • Need media? We are selling PBS tubes for $20 per pack of 10 tubes. 
    • Want to use your own? Please use at least 3 mL of PBS or saline in leak-proof tubes that are 10 mL or smaller and do not have any additives.
  • Once samples are received in the laboratory, they become the property of the laboratory and will not be returned to the client for any reason.


Supplies are available from the Colorado Department of Agriculture Animal Health Laboratory to accredited veterinarians. Some quantities may be limited and all prices are subject to change. Please let us know in advance if you have a large order to place so that we may have the full quantity of the item you need.

AHL Supplies

Supplies can be ordered by phone, fax or email:

Phone: 303-869-9230
Fax: 303-869-9232
AHL also supplies EIA Test Records, Brucellosis Test Records, Tuberculin* and more! Call or email us for more information.
*Due to the significant increase in shipping costs we now charge a $10 flat rate to ship tuberculin from this lab. We now maintain a limited supply of tuberculin. We can place an order with NVSL to ship at no charge to you, however shipping from Iowa will take longer than shipping directly from this lab.

Testing Services and Fees

There is a 24/7 sample drop box located at the northeast corner of the building. Please place samples in a cardboard box before placing in the drop box. If left in a bag samples may break.

When sending samples via FedEx, UPS or USPS please package samples carefully so they are not damaged in transit. Submissions to the laboratory should comply with applicable biosafety regulations. Submissions to the laboratory must be accompanied by a proper submission form for all samples except EIA mobile submissions.

Testing charges will be billed to the veterinarian submitting the samples. There is no additional charge for samples originating from out-of-state.

Testing Services and Fees


The Animal Health Lab now offers a NEW electronic spreadsheet available as an Excel file or Google sheets file or our standard sample submission form. The lab must receive one or the other via email (email preferred) or a hard copy with samples for general serological or molecular test requests. If submission documents will be sent electronically and NOT included with samples, please label samples clearly so that they may be distinguished and matched up to the submission documents upon check in.

Be sure to fill out the submission documentation completely. Please note that the spreadsheets have separate tabs for animal info and test request/owner info. Any missing information on the submission documentation will lead to a delay in testing. Please take the time to check that all required information is on the submission form before mailing samples in.

    A VS 10-11 form (unless using the EIA Mobile App) must accompany EIA samples and a VS 4-33 must accompany all official Brucella abortus, Brucella suis and Brucella melitensis testing. All USDA Forms are available at the link below.


    Submission Protocol

    The following submission protocols are provided so that the highest quality sample can be obtained. Collecting the sample the right way the first time can save a lot of time and hassle. Following the protocols will help to ensure that samples are of sufficient volume and quality for testing.

    Johne's Fecal PCR Submission Protocol

    Sample Submission Protocol

    • Use this protocol when submitting samples for serological testing such as Anaplasmosis, Brucella abortus, B. canis, B. melitensis, Bluetongue, Bovine Leukosis, Caprine Arthritis Encephalitis, Equine Infectious Anemia, and Pseudorabies.
    • General Serology Submission Protocol

    Tritrichomonas foetus Sample Collection Protocol


    Questions Regarding Supplies

    Q:   Where do I get Tuberculin?

    A:   Right here at the AHL, just give us a call at 303-869-9230. Please note: due to the significant increase in shipping costs we now charge a $10 flat rate to ship tuberculin from this lab. We now maintain a limited supply of tuberculin. We can place an order with NVSL to ship at no charge to you, however shipping from Iowa will take longer than shipping directly from this lab.

    Q:   Where do I get Equine Infectious Anemia (EIA) submission forms?

    A:   Right here at the AHL, just give us a call at 303-869-9230. Ask us about our free EIA mobile app.

    Q:   Where do I get OCV (orange) and Bright (silver) tags?

    A:   The Animal Health Laboratory supplies both of these tags. Please call 303-869-9230 to order these items.

    Q:   Where do I get books of Interstate Certificates of Veterinary Inspection?

    A:   The Animal Health Laboratory still supplies books of Interstate Certificates of Veterinary Inspection for $75/book of 25 certificates. Please call 303-869-9230 to order. If you are interested in using electronic certificates of veterinary inspection call the Colorado State Vet's Office at 303-869-9130.

    Questions Regarding Sample Submission

    Q:   How much blood do I need for testing?

    A:   Please provide at least 2 ml of serum for serological tests. If you are requesting multiple tests only one tube with 2 ml of serum is generally necessary to handle all of the tests requested.

    Q:   What kind of tube can I use?

    A:   For most of the serological testing done here, any red top or serum separator tube, 10 cc or less is acceptable.

    Q:   How long can I expect to wait for results?

    A:   See Testing Services and Fees for testing turn-around-times.

    Questions Regarding Public Health Issues

    Q:   What do I do if I've found a dead squirrel or bird?

    A:   The Animal Health Laboratory does not handle any type of animals directly. Please contact the Colorado Department of Public Health regarding any dead animal you suspect of having died of suspicious causes. Their animal helpline is (877) 462-2911.

    Contact Us

    Tiffany Brigner
    Laboratory Manager
    300 S. Technology Ct. Broomfield CO 80021

    Additional Resources

    CSU Logo.


    CSU Diagnostic Laboratory


    USDA APHIS Forms

    A2LA Accreditation

    Scope & Certificate Of Accreditation To ISO/IEC 17025:2017

    A2LA Accreditation

    The Colorado Department of Agriculture's Animal Health Laboratory (CDA-AHL) is accredited to ISO/IEC 17025:2017 and the OIE Quality Standard and Guidelines for Veterinary Laboratories: Infectious Disease, 2008 through the American Association for Laboratory Accreditation (A2LA). These internationally recognized standards set stringent requirements for operating quality systems in testing laboratories.

    If you would like to find out additional information on A2LA's accreditation program please visit A2LA.


    A2LA accredited symbol

    In January 2006, the Colorado Department of Agriculture's Animal Health Laboratory (CDA-AHL) gained an elite accreditation from the American Association for Laboratory Accreditation (A2LA). The Animal Health Lab attained ISO/IEC 17025:2017 accreditation which is an internationally recognized standard that sets stringent requirements for operating quality systems in testing laboratories. Every other year the lab participates in an on-site assessment to maintain this accreditation.